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KMID : 1094720150200050942
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 5 p.942 ~ p.947
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Physicochemical characterization of gelatin-immobilized, acrylic acid-bacterial cellulose nanofibers as cell scaffolds using gamma-irradiation
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Lim Youn-Mook
Jeong Sung-In
Shin Young-Min
Park Jong-Seok
Gwon Hui-Jeong
Nho Young-Chang
An Sung-Jun
Choi Jong-Bae
Jeong Jin-Oh
Choi Jae-Won
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Abstract
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Bacterial cellulose (BC) has been shown to have a high-burst pressure, high-water contact, and ultrafine highly nanofibrous structure similar with that in a natural extracellular matrix (ECM). In the present study, we developed a BC-based functional scaffold for tissue engineering using radiation technology. BC was generated by Gluconacetobacter hansenii TL-2C. Acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation. The characterization of the scaffold was performed by scanning electron microscopy, ATR-FTIR spectroscopy, a toluidine blue O assay, and 2,4,6,-trinitro-benzensulfonic acid assay. AAc was grafted on the BC under gamma-ray irradiation. Gelatin was chemically conjugated on the AAc-BC scaffolds through EDC chemistry. The morphology of the modified BC nanofibers did not change, while representative features of AAc and gelatin were maintained. The adhesion and spreading of human mesenchymal stem cells was improved on the gelatin-AAc-BC nanofibers compared to unmodified BC and AAc-BC nanofibers. Our results suggest that gelatin-immobilized BC nanofiber scaffolds can be a promising way to fabricate three-dimentional, nanofibrous scaffolds that accelerate cell behavior for biomedical applications.
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KEYWORD
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bacterial cellulose (BC), acrylic acid (AAc), gelatin, gamma-ray irradiation, tissue engineering
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